Chromatography Is a way of separating the parts of a mix of a gas or liquid solution containing different substances. By way of instance, pen inks are usually composed of different colors. The various bonding properties of each molecule type is exploited. Chromatography is used in both qualitative and quantitative analysis of both inorganic and organic samples. This technique utilizes two types of materials
- Mobile phase
A liquid or gas which transports the solution being analyzed through another material water, rubbing alcohol are examples.
- Stationary phase
The liquid or solid through the tested substance is carried coffee filter paper, paper towel are examples.
It Is vital for the various substances in the solution of have different properties like molecule size or another ability to dissolve in a solvent. The static phase will absorb or slow down distinct elements of the analyzed solution to various degrees creating layers because the elements of the solution are split. Chromatography was devised by the Russian botanist, Mikhail Tsvet. Chemists use this procedure to identify unknown substances by dividing them in the different molecules which make them up. Paper Chromatography can be demonstrated with a very simple demonstration.
- Water soluble pencils or markers of different brands or colors
- Strips of paper towel
- rubbing alcohol
- nail polish remover
- Straw or pencil or pencil
These different molecules have different Characteristics like size and solubility. Solubility is a molecule’s ability to dissolve in a particular solvent like alcohol, water or nail polish remover. Due to their distinct attributes, each molecule travels at a different rate when pulled across the bit of paper toweling from the solvent. Thus, all the pigments which make up an ink sample are separated out.
Unknown substances Left at a crime scene can be recognized by separating the molecules which make them up. Matching this unknown chromatogram into chromatograms of known substances can help identify the unidentified substance giving a hint to the crime. The ink used in a note left at a crime scene can be recognized through paper chromatography. The chromatogram showing the parts of the ink used in the note could be contrasted to chromatograms of known inks identifying the type and brand of ink used providing a hint to the offense. It is used in the sequencing of both DNA and RNA. Paper chromatography is used as a qualitative analytical chemistry technique for separating and identifying colored mixtures like pigments. It is used in scientific research to determine unknown organic and inorganic chemicals from a mix. So as to obtain a pure sample every part of the split mixture, the separated spots on the dried and finished chromatogram can be cut out and re-dissolved.